Synthesis of magnetic electroactive nanomotors based on sodium alginate/chitosan and investigation the influence of the external electric field on the mechanism of locomotion

In this paper, we report a novel electric-driven Janus nanomotor (JNMs) based on SPIONs nanoparticle decorated with chitosan (Cs) and sodium alginate (Na/Alg) using the Pickering emulsion method. The JNMs dispersed in aqueous media exhibit linear trajectories under DC electric field, and the driving force is attributed to the self-electro-osmotic mechanism and surface modifications. This study offers an approach to remotely control the motion modes of the JNMs, including start, stop, directional and programmable motion, which can be advantageous for various application scenarios. The diffusion coefficient and velocity of the JNMs were investigated through mean square displacement analysis for single particle of JNMs, both in distilled water and in the presence of different di and trivalent metal cations (Fe3+, Al3+, Ba2+, Ca2+ and Mg2+) as crosslinking agents, as well as monovalent salts (LiCl and KCl). The results revealed that the motion of JNMs was fastest in the presence of Fe3+ as crosslinker agent (about 7.2181 μm2/s) due to its higher charge than equimolar Na+ . Moreover, it was demonstrated that increasing the ionic strength led to relatively higher speeds of JNMs, as the solution polarity increased and, as a result, the driving force of electro-osmoesis enhanced.

www.nature.com/scientificreports/ nanomotors have received considerable attention, especially in biomedical and pharmaceutical fields, due to their fuel-free propulsion, remote controllability, and harmlessness to cells and tissues 14,15 . This strategy not only provides the power movement of micro/nanomotors in a noncontact manner but also is able to concentrate active micro/nanomotors at predefined locations. In another instance, a new driving mechanism was reported that allows more switchable motion control on the orientations, alignment speeds, and directions of semiconductor silicon nanowires in the presence of an external electric field with simple visible-light exposure. The nanowire can be toggled instantly between parallel and transverse alignment to the E-field as a result of the interaction between the in-phase (real-part) electric polarization of the particles and the electric field 16 . Wu et al. reported the synthesis of self-propelled magnetic micromotors based on polystyrene spheres anisotropically coated with Cr/Ni/Au layers and investigated their propulsion under magnetic/electric hybrid power. They showed that the combination of a rotating magnetic field and an electric field could enhance micromotor mobility and direction control. In addition, this Janus microspheres could identify apoptotic cell among viable and necrotic cells based on their dielectrophoretic difference 17 . Xiao and coworkers reported fully fuel-free hybrid nanomotors that exhibited efficient propulsion in the presence of UV light and AC electric fields without adding any chemical fuel. They described bi-segmented hybrid Janus nanomotors in which both segments of TiO 2 and Pt respond to UV and electrical fields. Since the two hemispheres of the TiO 2 -Pt micromotor polarize very differently under the electric field, the induced charge electroosmosis (ICEO) is produced with different magnitudes on the two surfaces, giving rise to an asymmetric flow that moves the particle forward. Both mechanisms, self-electrophoresis under UV light and induced-charge electrophoresis (ICEP) under AC electric fields, are well-documented and propel the particle towards the same direction, which resulted in up to 90% faster than simply adding up the speed powered by either source 18 . Among the proposed micro/nanomotors powered by an electric field, polymer-based micro/nanomotors are of great significance for the ease of processing and facile functionalization, holding colossal potential for bioapplications 19 . Recent progress in this field was focused on metallodielectric patchy polymeric particles that enable active systems to perform sophisticated tasks in the future, such as selective cell treatment. Metallodielectric patchy particles are constructed from a metallic (gold) lobe and a dielectric (polymer) lobe. The charges responding to the AC field can propel the patchy particle under the ICEP mechanism with the dielectric lobe facing forward. These colloidal molecules exhibit controllable and switchable dynamic behaviors, such as propulsion, steering, and reconfiguration 20 . Recently has been shown that asymmetric metal patches on the surface of spherical colloids, in this case, polystyrene microspheres with metallic patches, can self-propel along non-linear helical trajectories powered remotely by an alternating current (AC) electric field by ICEP mechanism 21 .
In practice, electric motion can be generated by either the potential energy produced with ICEP and selfregulated electroosmosis from the DC or the AC electric field. ICEP is the most commonly used method to drive asymmetric micro/nanomotors with different shapes or polarizabilities in the electric field. The polarization of the asymmetric micro/nanomotors under an electric field induces an asymmetric accumulating countercharge in the electric double layer (EDL) around the motors and generates an electro-osmotic flow around these particles. The asymmetric EDL could be due to the different polarizabilities of the existing materials in the structure of micro/nanomotors. The more polarizable part of the EDL would accumulate more charges and form a stronger induced charge electro-osmosis (ICEO) to drive the asymmetric micro/nanomotors 22 . Practically, the motion control, especially acceleration or deceleration of velocity based on demand, can be directly influenced by tuning the strength of the electric field and the direction of movment is determined by the type of motors as well as the direction of the field 23 . Combining the electro-osmosis and the IECP caused by the asymmetric electrical polarization of the nanomotors under an electric field provides a strategy to synthesize electrically responsive nanomotors based on electroactive polymers 24 . For the first time, a new type of defective electric-driven micro/ nanomotor with an asymmetric shape proposed, which can perform direction-switchable 2D motion behavior and bioinspired 3D helical motion behavior for cargo transportation. These nanomotors can be widely applied for various purposes, e.g. biomedicine, micro/nanosensors, and micro/nanomechanical systems. It is reported that the defective golden nanomotor can demonstrate controllable self-dielectrophoresis and ICEP motion behaviors in different frequency range through generating an asymmetric field gradient caused by structural deficiency 15 .
Herein, we synthesized fuel-free Janus micro/nanomotors (JNMs) powered by electrical fields. The JNMs were fabricated using Pickering emulsion consisting of a magnetite core (Fe 3 O 4 NPs) anisotropically coated with chitosan (CS) and sodium alginate (Na/Alg). In This system, as an innocuous approach that is easy to operate, electric field has been used to produce the requisite propulsion. When the electric field is applied, the existing positive ions go toward the cathode, and the negative charge gathered around the sodium alginate polymer causes electroosmotic pressure, which causes the movement of these nanoparticles toward the opposite electrode. These micro/nanomotors exhibited a rapid oscillatory motion between the two electrodes which every single particle is propelled toward the anode electrode by a electro-osmotic mechanism. One of the intriguing advantages of these micro/nanomotors is that a controllable manipulator could be used to freely and easily start and stop the power source, allowing a switchable mode of movement of the micro/nanomotors in the presence or absence of an electrical field. Furthermore, such micro/nanomotors can be programmed to move along the intended trajectory in a controllable manner by adjusting the direction of the anode and cathode electrodes.
Synthesis of acrylated sodium alginate. Synthesis of low molecular weight alginate. Degradation of high molecular weight sodium alginate to low molecular weight sodium alginate was prepared using a modified procedure based on previous reports 25 . A 1000 mL round bottom flask was charged with 7.5 g of high molecular weight sodium alginate in 500 mL of distilled water (1.5% w/v). Then 3.5 g of ascorbic acid and 5 mL of pyridine were added to the reaction medium, and the mixture was stirred at room temperature for 15 min. The flask was fitted with a rubber septum and placed under Ar atmosphere; the resulting solution was placed in an oil bath and heated up to 80 °C. Followed by adding 50 mL of 30% hydrogen peroxide to the reaction via syringe, the reaction mixture was then stirred at 80 °C for 2 hrs. The low molecular weight sodium alginate powder was obtained by lyophilizing from water and analyzed by aqueous gel permeation chromatography (aq-GPC).
Synthesis of tetrabutylammonium alginate (TBA/Alg). In the next step, for better dissolution of the polymer in non-aqueous solvents for acrylation, cation exchange with tetrabutylammonium hydroxide (TBAOH) was performed using previous methods 25 . 250 mL round bottom flask was charged with 100 mL solution of ethanol/0.6 M HCl in water (1:1 v/v), then dried low molecular weight sodium alginate (1 g) was added to the solution. The flask was fitted with a rubber septum and the mixture solution was stirred at 4 °C for 24 hrs. The product was filtered by vacuum and purified by recrystallization in ethanol and then concentrated under reduced pressure to produce low molecular weight alginic acid. A dilute solution (4% w/v) of tetrabutylammonium hydroxide (TBAOH) was added dropwise to the solution of low molecular weight alginic acid in water (3% w/v), until the pH value was adjusted between 7 and 9 by controlling with pH-meter. The reaction mixture was stirred at room temperature for 24 hrs. The final product (TBA/Alg) was precipitated in ice-cold ethanol, centrifuged and washed with ethanol (3 times). The dry product was characterized by 1H-NMR in D 2 O 25 .
Synthesis of acrylayed sodium alginate (AcNa/Alg). Acrylayed sodium alginate (AcNa/Alg) was prepared according to Vitaliy and Ronald procedure with slight modifications 25 . Briefly, the synthesized TBA/Alg (666 mg, 4.16× 10 -2 mmol) and triethylamine (1.11 mL, 8 mmol) were dissolved in 6 mL DMSO with 1% tetrabutylammonium fluride (TBAF) at 0 °C. Acryloyl chloride (0.48 mL, 6 mmol) was then added dropwise to the mixture, and the stirring was continued at room temperature for 48 hrs, under a dry nitrogen atmosphere. Afterward, the acrylated tetrabutylammonium alginate (Ac TBA/Alg) was obtained by pouring the reaction solution into cold water and then filtering the precipitate. By adding sodium carbonate solution to the suspension of AcTBA/Alg in water, the water-soluble acrylated sodium alginate (AcNa/Alg) was produced, which was isolated by precipitation in ice-cold ethanol, washed with water, and then lyophilized from water. AcNa/Alg was dried in a vacuum oven at 40 °C overnight for further characterization with 1HNMR (in D 2 O).

Synthesis of Cs-Fe 3 O 4 -Na/Alg Janus nanoparticles. Synthesis of magnetite nanoparticles half-coated
with chitosan. The method reported in a previous work 26 (S1.1-S1.6) was used to synthesize Cs-cisplatin-Fe 3 O 4 Janus nanoparticles. To decorate the half-part of the NPs with hydrophilic groups, half of the prepared SPION was first entrapped in wax droplets using the Pickering emulsion method, and then the remaining bare surfaces were modified with APTES. In the next step, the attachment of chitosan to the bare surface of SPIONs was required, for which cisplatin was selected as a linker to conjugate chitosan to the surface of SPIONs. The reaction between the chloride ligand of cisplatin and the amine groups of APTES via nucleophilic substitution was used to react cisplatin with the remained amine groups of APTES. To achieve chitosan-coated Janus NPs, all of the half-masked NPs (Cisplatin -APTES-Fe 3 O 4 -wax microspheres) obtained in the preceding step were dispersed in deionized water and mixed with chitosan. The amino functional group of the chitosan replaced the chlorine group functionalized particles via the ligand exchange method. In the next step, the chitosan-Cisplatin APTES-Fe 3 O 4 /wax droplets obtained were filtered and washed with deionized water to remove free particles and excess chitosan. All these procedures were performed at room temperature. Finally, the unmasking Cs-cisplatin-Fe 3 O 4 Janus nanoparticles (JNPs) were collected after washing them with chloroform at 70 °C using the bain-marie technique.

Functionalization of the bare side of Cs-cisplatin-Fe 3 O 4 JNPs with 3-aminopropyl triethoxysilane (APTES).
Cscisplatin-Fe 3 O 4 nanoparticles (300 mg) were dispersed in 5 mL ethanol then APTES (0.46 mL, 2 mmol) and distilled water (0. 5 mL) were added to the dispersion, and the mixture was kept stirring at room temperature for 24 h. The resulting mixture was washed three times with ethanol to remove the unreacted amount of APTES, and the amine-decorated Cscisplatin-Fe 3 O 4 nanoparticles (Cs-cisplatin-Fe 3 O 4 -APTES) were kept in distilled water for further reactions.
Synthesis of Janus (Cs-Fe 3 O 4 -Na/Alg) nanoparticles. As the first step, 2g (0.125 mmol) acrylated sodium alginate (AcNa/Alg) was dissolved in distilled water (70 mL) and the solution was stirred at room temperature for 30 min (solution 1). Cs-cisplatin-Fe 3 O 4 -APTES (0.5 g) was dispersed in distilled water (5 mL) under ultrasonic irradiation for 10 min and rapidly added to the solution 1, and kept stirring at room temperature for 72h. The obtained Janus nanoparticles coded as (Cs-Fe 3 O 4 -Na/Alg) JNPs were separated using external magnetic field and were washed five times with distilled water and kept in distilled water. www.nature.com/scientificreports/ Synthesis of fluorescently labeled Janus (Cs-Fe 3 O 4 -Na/Alg) nanoparticles. Synthesis of fluorescein-labeled cisplatin (FL-CisPt). 33.23 mg (0.1 mmol) fluorescein was added to 4 mL distilled water and allowed to dissolve completely in water. Then 11.509 mg (0.1 mmol) NHS was added to the reaction mixture at room temperature and stirred for 5 min. Followed by the addition of 28.755 mg (0.15 mmol) EDC in 2 mL H 2 O, the reaction mixture was stirred at room temperature for 4 h, then (0.1 mmol) cisplatin was added to the mixture and the reaction media was stirred at room temperature for 24 h. After completing the reaction the product was extracted with DCM (5×60mL). The combined organic phases were dried over Na 2 SO 4 and concentrated under vacuum to produce a yellow viscous liquid with a 55% yield. For fluorescence imaging, the dye-tagged cispaltin was dissolved in dichloromethane and then dried on the glass slides. . Before measurement, the nanoparticles were sonicated for 10 min to prepare a homogeneous solution and finally, the size distribution of nanoparticles was measured at 25 °C. Zeta potential is an important index of surface charge which represents particle stability in dispersion. As mentioned above, after dispersing and sonicating the nanoparticles, zeta potential analysis was performed by DLS. The dried size and morphology of Janus Cs-Fe 3 O 4 -Na/Alg nanoparticles were measured using transmission electron (TEM) (Tecnai G2 F20 STwin). The nanoparticles were dispersed in deionized water and a drop of nanoparticles suspension was placed on a carboncoated grid. The grid was completely dried and used for TEM analysis (150 kV). The weight average molecular weight ( M w ), number average molecular weight ( M n ), and polydispersity index (PDI) of sodium alginate after degradation were determined by using the gel permeation chromatography (GPC) (PL-EMD 950). The motion behavior of Cs-Fe 3 O 4 -Na/Alg JNMs were recorded using a Motic AE 31 optical microscope by applying an electric field in distilled and deionized water media. The movement of the nanomotor is toward the anode electrode; therefore, the direction of the motion will be reversed by switching the anode and cathode. Electrical control of the movement of micromotors is done by applying "on" and "off " electric current with different time intervals and recording with a camera. It is necessary to mention that the length of time of being "off " and "on" is identical, and it is equal to the number that is expressed by the word "pulse" in videos. As well as, a transient change in the amplitude of the electric field from the baseline 0 V to 1.5 V and 3Vwas applied and the JNMs movements were recorded. Next, the effect of crosslinking ions on the physical properties and velocity of JNMs was evaluated by adding an equimolar solution of divalent and trivalent metal cations (BaCl 2 , CaCl 2 , MgCl 2 , FeCl 3 or Al 2 (SO 4 ) 3 ) to the pristine JNMs and their motions were recorded under an electric field with an amplitude of 1.5 V. For this purpose, a solution of each salt with a concentration of 1 mmol in 10 mL distilled water was prepared. Next, each solution was transferred to a beaker containing a dispersion of 5 mg Cs-Fe 3 O 4 -Na/Alg in 10 mL distilled water, and the mixture was stirred for 30 min. The highly diluted solutions were prepared by adding a drop of every solution into 9 mL of distilled water to acquire high-quality trajectories. The framerate of all videos was 66 frames per second and the magnification of the microscope was 400x. After that videos were magnified 4 times for more reliable tracking.

Results and discussion
Synthesis and characterization of magnetite-based chitosan/sodium alginate JNPs. New technologies are being developed to improve targeted drug delivery using catalytic nano/micromotors. By modifying their structures, performance can be enhanced and adverse effects minimized. This paper focuses on developing a new manufacturing method for swimming devices with two key features: (i) the ability to move without chemical fuel; and (ii) directional control through a biocompatible polymer coating. The aim is to create a Janus nanomotor using biodegradable polymers like sodium alginate (Na/Alg) and chitosan (Cs) by the Pickering emulsion method, that moves with an electrical field and electro-osmosis mechanism. These motors offer a safer www.nature.com/scientificreports/ option for biomedical applications such as drug delivery, micro-manipulation, and bio-imaging-guided procedures. For this purpose, SPION nanoparticles were synthesized and trapped in wax microspheres according to Fig. S1, Step 1 26 . The non-coated faces of nanoparticles were modified with APTES (Fig. S1, Step 2) 26 . APTES-Fe 3 O 4 wax microspheres can be easily dispersed in water media due to the presence of amine functional groups on the half surface of the nanoparticles after the reaction of SPION with APTES. In the next step, our target is the chemically linking chitosan to the non-protected surface of SPION nanoparticles. For this purpose, cisplatin was selected as a linker and conjugated to the bare surface of modified SPIONs via the nucleophilic substitution reaction between the chloride ligand of cisplatin and the amino groups of nanoparticles (Fig. 1a). Energy-dispersive X-ray spectroscopy (EDX) was used to identify the chemical compositions of wax-free cisplatin-Fe 3 (Fig. S4) and subsequently reacted with amine groups already available on the Fe 3 O 4 surface after the APTES reaction (Fig. S5). Figure S4 illustrates the fluorescence microscopy images of cisplatin before and after incubation with fluorescein. Cisplatin aggregates are apparent in their pure form, while, after treatment with fluorescein, a good dispersion of the FL-CisPt sample discolored to the green color is observable. The formation of the FL-CisPt was confirmed by UV absorption spectra analysis and compared with the pristine cisplatin and fluorescein (Fig. S6). The characteristic absorption peaks of FL-CisPt at 207 and 494 nm correspond to the fluorescein and platinum segments, respectively, with a slight shift. In the next step, fluorescein-tagged and -untagged cisplatin were reacted with APTES-Fe 3 O 4 JNPs (Fig. S5), and their connection was evaluated by Fluorescence spectroscopy. The fluorescent color observable in the fluorescein-tagged sample proved the tagging reaction. Due to the presence of SPION in the fluoresceintagged SPION compound, its characterization by UV spectroscopy is impossible.
The main purpose of using cisplatin in this work was to bind chitosan to the half-surface of SPION. Accordingly, chitosan amine groups attached to the cisplatin remained chloride groups via the ligand exchange method (Fig. 1a). The mean diameter of Cs-CisPt-Fe 3 O 4 -wax microparticles was determined using optical microscopy and Image J software. Analysis of 100 particles yielded a mean diameter of 4.55 ± 1.43 µm (Fig. S7). Cs-cisplatin-Fe 3 O 4 -wax microparticles were washed off with chloroform to remove the wax, dry in the oven at 40 °C, and then analyzed by FT-IR spectroscopy to characterize the produced chitosan half-coated nanoparticles. The typical FT-IR spectra of pure chitosan and Cs-cisplatin-Fe 3 O 4 JNPs are shown in Fig. S8. The peak at 3300 to 3500 cm −1 was related to the stretching vibrations of chitosan hydroxyl groups. The bsorption peaks at 1630 and 1531 cm −1 are due to the N-H bending vibration of protonated amine (−NH 2 ) groups and C-H bending vibration of the alkyl groups, respectively. The anti-symmetric stretching vibration of C-O-C bridges and glucopyranose ring of chitosan were located at 1059 and 886 cm −1 , respectively. Cs-cisplatin-Fe 3 O 4 JNPs demonstrate the characteristic peaks of cisplatin at 699 and 756 cm −1 , as well as all the chemical bonds of pure chitosan, but with small displacements. This displacement indicates the chemical reaction between cisplatin found at the half-surface of modified nanoparticles and chitosan. Fluorescein was reacted with chitosan of the Cs-cisplatin-Fe 3 O 4 JNPs via EDC/NHS chemistry to prove the presence of chitosan in half of the structure of Janus nanoparticle. The fluorescein-tagged nanoparticles exhibited a bright green light emission of the fluorescein segment at one-half of the nanoparticles under a UV lamp (Fig. S9).
Till here, half of the surface of magnetite nanoparticles was covered by chitosan. For the synthesis of Janus nanoparticles containing chitosan and alginate domains, the wax removal from the Cs-cisplatin-Fe 3 O 4 -wax microparticles was performed by chloroform. Then by the reaction of Cs-cisplatin-Fe 3 O 4 NPs with acrylated alginate, the final CsFe 3 O 4 -Alg JNPs were prepared as shown in Fig. 1b. The total synthesis of acrylayed sodium alginate (AcNa/Alg) has been achieved in five steps, as illustrated in Fig. 2.
Due to the low solubility of pristine alginate in organic solvents, at first, high molecular weight alginate was depolymerized into shorter fragments using hydrogen peroxide (H 2 O 2 ) at 80 °C. In the next step, Alginate-TBA was synthesized through the cation exchange reaction via the reaction of the LMW-alginic acid with tetra butyl ammonium hydroxide (TBA-OH) to improve its solubility in DMSO. Modification of TBA Alginate occurred when hydroxyl groups of alginate reacted with acryloyl chloride in DMSO and produced acrylated TBA alginate (AcTBA/Alg). Neutralization with sodium carbonate resulted in the formation of acrylated sodium alginate. Measuring molecular weight after depolymerization reaction (i.e. 1st step) using aq-GPC showed that weight average molecular weight (MW) of the polymer was 24,000 g/mol with polydispersity index (PDI) of 2.23. The product of LMW-sodium alginate with HCl obtained from the second step was characterized by FT-IR spectroscopy. As illustrated in Fig. S10, the stretching vibrations of asymmetric and symmetric bands of carboxylate www.nature.com/scientificreports/ anions are located at 1620, 1432, and 1300 cm −1 , respectively, and the peak appeared at 3400 cm −1 corresponds to stretching vibrations of hydroxyl groups. The formation of alginic acid was confirmed by the shift of the characteristic carbonyl group peak from 1620 to 1726.08 cm −1 , which is due to the conversion of sodium alginate carboxylate groups to alginic acid carboxylic acid groups. The positions of the other peaks are nearly identical for the two above-mentioned alginate derivatives. www.nature.com/scientificreports/ In the third step, the LMW-alginic acid was reacted with TBAOH to produce TBA alginate (TBA/Alg). 1 H NMR and FTIR were applied to evaluate the chemical composition of TBA/Alg ( Fig. 3a and b, respectively). From the 1 H-NMR spectrum shown in Fig. 3a, the structure of TBA/Alg has been confirmed with peaks appearing between 3.4 and 5.00 ppm, which represent alginate backbone hydrogens and four peaks from butyl hydrogens on TBA (0.70 ppm, 3H, t; 1.27 ppm, 2H, m; 1.55 ppm, 2H, m; 3.20 ppm, 2H, m). The FT-IR spectrum of TBA/Alg (Fig. 3b) exhibited almost all the FT-IR peaks of alginic acid; plus, two new absorptions at 2956 and 2872 cm −1 assigned to the C-H stretching vibrations of the saccharide structure and tetra butyl ammuniom segments. The shifting of carbonyl stretching frequency to the lower wavenumbers (1603 cm −1 ) indicated a successful cation exchange reaction and the reconstitution of carboxylate groups (Fig. 2b).
Functionalization of TBA/Alg with acrylate groups was carried out by reacting TBA/Alg with acryloyl chloride in the presence of triethylamine under anhydrous and inert conditions, as illustrated in Fig. 2. Successful acrylation is confirmed by the appearance of 1 H-NMR peaks at δ = 5.8 (i), 6.11 (j), and 6.37 (k) ppm, corresponding to the hydrogens on the acrylate groups (Fig. 4a). This spectrum illustrates all characteristic peaks of TBA/ Alg between 0.7 and 5.00 ppm. FT-IR analysis was used to elucidate the structure of acrylated TBA alginate. The appearance of two new absorptions at 1724 and 1629 cm -1 , corresponding to the C=O and C=C stretching bonds of AcTBA/Alg acrylate groups besides the pre-existing peaks of TBA/Alg (Fig. 4b) indicated the successful   www.nature.com/scientificreports/ esterification of alginate by acryloyl chloride. The results illustrated that grafting the hydrophobic acryloyl groups on the alginate backbone chain could destroy the original intramolecular hydrogen bonding of alginate and consequently enhance its molecular flexibility 28 . Finally, AcTBA/Alg was reacted with sodium carbonate, and then lyophilized from water. The dry product was characterized by 1 H-NMR (in D 2 O) and FT-IR spectroscopy. The retained alginate peaks can be seen at 3.7-5.0 ppm, and the TBA fraction was no longer present, as it was removed during neutralization with Na 2 CO 3 (Fig. 5a). The disappearance of the peak at 1209 cm -1 is related to the C4 skeletal stretching of the butyl groups and the acrylate carbonyl group in the FT-IR spectrum of AcNa/Alg spectrum, which indicates the successful neutralization with Na 2 CO 3 (Fig. 5b).
For the synthesis of chitosan/sodium alginate Janus nanoparticles (Cs-Fe 3 O 4 -Na/Alg), at first, Cs-cisplatin-Fe 3 O 4 JNPs were reacted with APTES and then, the reaction between amine functional groups of Cs-cisplatin-Fe 3 O 4 -APTES JNPs and acrylates functional groups in AcNa/Alg results in the preparation of the Janus nanoparticles (Fig. 1b). FT-IR spectrum of (Cs-Fe 3 O 4 -Na/Alg) is shown in Fig. 6a and compared with their pure polymer sources. The strong O-H stretching peak appears at 3400 cm −1 , which is also observed in pure chitosan spectra. At 2923 cm −1 there is a strong peak related to the C-H stretching, which belongs to the alkane groups of the chitosan rings, alginate, and APTES. The ether and ester groups of chitosan and alginate can be distinguished as the peak at 1071 cm −1 corresponding to the C-O stretching vibrations. C-N stretching and N-H bending vibrations of amine groups in chitosan are significant as peaks at 1100 and 1600 cm −1 . The appearance of the absorption bands around 1680, 1416, and 1306 cm −1 attributed to stretching vibrations of asymmetric and symmetric bands of Alg carboxylate anions, respectively confirmed the binding of Alg to nanoparticles, and the preparation of Cs-Fe 3 O 4 -Na/Alg JNPs.   www.nature.com/scientificreports/ Thermogravimetric analysis (TGA) and derivative thermogravimetric analysis (DTG) were used to determine the amount of chitosan and alginate linked on the surface of NPs. For this purpose, the Janus nanoparticles before alginate adding (i.e. Cs-cisplatin-Fe 3 O 4 JNPs) and after that (i.e. Cs-Fe 3 O 4 -Na/Alg JNPs) were analyzed by TGA (Fig. 6b). In the TGA curve of Cs-cisplatin-Fe 3 O 4 sample, the first notable weight loss occurred by heating from room temperature to 110 °C which is related to the evaporation of trapped and physisorbed water from the sample (Fig. 6b-I). The second distinct weight loss starts at 217 °C related to the decomposition of conjugated chitosan 29 . The chitosan mass loss is about 23.41%, which means the inorganic fraction to polymeric fraction (chitosan) in the first Janus nanoparticles is 76.59%:23.41%. TGA/DTG thermograms of the second Janus nanoparticles confirms the presence of chitosan and Na/Alg domains on the Janus Cs-Fe 3 O 4 -Na/Alg surface. The TGA thermogram of Cs-Fe 3 O 4 -Na/Alg JNPs exhibited a gradual degradation from 130 to 650 °C (Fig. 6b-I), while, DTG illustrated the first decomposition step in the range of 130-210 °C with a maximum at 184 °C assigned to sodium alginate degradation and a second degradation in the 205-314 °C region with a maximum at 254 °C ascribed to degradation of chitosan and sodium alginate 30,31 (Fig. 6b-II). According to the TGA curve of Cs-Fe 3 O 4 -Na/Alg, a weight loss of 2.37% was found for residual water. According to the DTG curve, the decomposition of sodium alginate was mixed with the degradation of chitosan before reaching the baseline. The thermal degradation continues gradually to 585 °C. In addition, a total weight loss of 46% was obtained due to the simultaneous degradation of chitosan and sodium alginate. The structure and morphology of the samples were characterized using transmission electron microscopy (TEM) (JEM-1011, JEOL, Japan) and scanning electron microscopy (SEM) (S4800, Hitachi, Japan). The SEM images of all the samples were taken after removing the wax from the surface of these nanoparticles. Figure 7a showed a spherical shape for APTES-Fe 3 O 4 JNP with an average particle diameter of approximately 48 nm. After coating APTES-Fe 3 O 4 JNP with Cs and Na/Alg, the average particle diameter increased to 56 and 67 nm, respectively, as shown in Fig. 7b and c. HR-TEM image of Cs-Fe 3 O 4 -Na/Alg JNPs in Fig. 7d displays the presence of alternated regions constructed from dark and bright areas probably related to Na/Alg and Cs polymers, respectively, and supported the formation of Janus structure with an average particle size in the range of 30-60 nm. The size obtained from TEM is in good agreement with that obtained from SEM.
In an attempt to further evaluate the nanoparticle properties, the particle size, polydispersity index (PDI), and surface charge of nanoparticles were determined by DLS. The size distribution of the  Table 1.
The average size of Cs-Fe 3 O 4 -Na/Alg JNPs was 132.9 nm with narrow size distribution (PDI = 0.099). Since the polymer chains on the surface of the JNPs swelled in water 32 , the diameter data resulting from the DLS study was much higher than that obtained from SEM and TEM. In the absence of any steric stabilization layer, the naked Fe 3 O 4 NPs show the zeta potential value of − 21.6 mV at pH 7.4, which showed relatively good stability (Table 1). This value decreased to − 13.9 when half of the surface of Fe 3 O 4 JNPs was reacted with APTES. The reaction of amine groups of APTES with cisplatin resulted in a new aminated sample; therefore, no significant change was observed in its zeta value. The zeta potential of Cs-cisplatin-Fe 3 O 4 JNPs is increased at pH = 7.4 due to the high number of amine functional groups present in the chitosan chains, which overcomes the negative charge of iron oxide. The shift of zeta potential n the three last samples was attributed to the presence of amine groups on the surface of the particles 33 . By shielding the other side of the Janus nanoparticle with Na/Alg, the zeta potential of Janus nanoparticles shows a reverse trend toward a negative datum and reaches − 30 mV. The decrease in the zeta potential of Alg-coated JNPs is due to the increase in the number of anionic polyelectrolyte www.nature.com/scientificreports/ COO¯. Thus, Cs-Fe 3 O 4 -Na/Alg JNPs with high zeta potential values provide a highly stable system by combining the steric and electrostatic hindrance due to the thick layer of alginate linked to JNPs.

Self-propelled motion of the nanomotors.
To investigate the motion of Janus nano/micro motors, a cell was designed and utilized as shown in Fig. 8. The cell was filled with deionized water and the motors were introduced between two Copper (Cu) electrodes. By applying an electric field through different voltages and pulses, charge separation occurred in the fluidic media containing Janus nanoparticles with at least one polarizable surface. As a result, the nanoparticles began to move in the opposite direction like a swimmer due to induced-charge electroosmosis (ICEO) 34 , where the sodium ions of the alginate domain moved towards the anode electrode while the charged solid particles generated flows around themselves and pushed the surrounding fluid. A digital electronic microscope connected to a digital camera was placed on the top of the chamber for observation purposes.
To investigate the effect of asymmetric ICEO flow around Janus nano/micro motors, we performed systematic experiments in a DC electric field (E) with different voltages and pulses (Videos S1-S5).
As shown in these videos, these JNMs exhibited electrically-driven with precisely steered start/stop switch, speed, and motion trajectory in aqueous media. The trajectories of the particles were analyzed by mean squared displacement (MSD) calculations. For this purpose, once the nanomotors started their motion under the electric  www.nature.com/scientificreports/ field, their movement was recorded with a camera at a speed of 10 frames per second. Tracking the nanomotors' motion was performed with a tracking tool programmed in MATLAB, and the MSD was calculated by Eq.   Table 2, the diffusion coefficient and velocity have a direct relation to voltage. So, an increase in their value is observed when the higher voltage is applied because the average (drift) velocity of ions in sodium alginate chains depends on the voltage of the source. Moreover, a significant decrease in the diffusion coefficient and velocity was observed by applying the pulse, and its MSD diagram became almost similar to the Brownian motion MSD diagram 35 .
In the next step, we investigated the effect of di-and trivalent metal cations (Fe 3+ , Al 3+ , Ba 2+ , Ca 2+ and Mg 2+ ) as alginate cross-linkers. The effects of this parameter were analyzed by considering the nanoparticle size and the nanomotors' speed. The nanoparticles crosslinked with divalent cations (Ba 2+ , Ca 2+ , Mg 2+ ) and trivalent cations (Al 3+ and Fe 3+ ) were prepared by dispersing Cs-Fe 3 O 4 -Na/Alg JNPs in the aqueous solutions containing either BaCl 2 , CaCl 2 , MgCl 2 , FeCl 3 or Al 2 (SO 4 ) 3 salts. The effect of Fe 3+ , Al 3+ , Ba 2+ , Ca 2+ , and Mg 2+ ions on sodium alginate domains was analyzed by determining the radius of nanoparticles through DLS method. According to the results obtained from the intensity mode of DLS, of aqueous suspensions of Janus nanoparticles, we can conclude that the process of binding of metal ions occurs via crosslinking of alginate by di and trivalent metal cations (Table 3).
By examining the size in terms of time, it is clear that the alginate part of Janus nanoparticles rapidly produces gel by creating intermolecular interactions with divalent and trivalent metal cations (BaCl 2 , CaCl 2 , MgCl 2 , FeCl 3 or Al 2 (SO 4 ) 3 ). The results show that the effect of trivalent ions on size reduction is greater than divalent ions and that of divalent ions are more efficient than monovalent ones.
We expected that the size of nanoparticles will affect the speed of the nanomotor cross-linked with each of these ions. For this purpose, the movements of nanomotors pre-suspended in different ions with equimolar concentration were recorded by the camera (Videos S6-S12), and their velocities were evaluated by MSD. As demonstrated in Table 4, adding divalent and trivalent ions with equal molar concentration to the aqueous solutions of nanomotors based on sodium alginate increases the speed of the nanomotors under the electrical field. This increase is higher for trivalent ions related to divalent ones because the trivalent ions provide more charge in the cell media and, as a result, create a higher driving force for moving the nanoparticles 37 . But what surprised us was the increase in speed by increasing the atomic weight in each group. Harlaftis et al. demonstrated that for spherical nanoparticles, the dielectrophoretic forces depend linearly on the particle volume, when external www.nature.com/scientificreports/  www.nature.com/scientificreports/ www.nature.com/scientificreports/ electric field, field density gradient, and frequency are constant 38 . So, according to this theory, in samples with identical ion charges, the increase in the speed according to the particle radius is logical. Figure 10a shows the trajectory of JNMs with different cationic agents as binders in an observation time of about 9 s. As can be seen, when Na + was replaced by Fe 3+ , the path traveled by JNMs increased from 6.4 to 24.99 μm. MSD analysis shows a semi-parabolic trend of nonlinear relationship, and its tangent slope gradually increases. Results obtained in the study showed that velocities of motion are 2.7767 ± 0.0025, 1.7459 ± 0.069, 2.061 ± 0.0073, 1.5005 ± 0.0048, 1.1081 ± 0.0012 µm/s for Fe 3+ , Al 3+ , Ba 2+ , Ca 2+ , and Mg 2+ respectively (Fig. 10b).
In the next step, the speed of nanomotors with some other monovalent ions was analyzed to evaluate the Harlaftis theory. For this purpose, potassium or lithium salts were added to the solution containing sodium alginate-based JNMs, in the same amount as di/trivalent ions and their movements were recorded (Videos S11 and S12). KCl and LiCl salts are not cross-linking agents, so the largest particle size is seen for nanomotors with these ions (Table 3). Interestingly, after Fe 3+ , the highest speed is for nanomotors containing Li and K (Table 4, Fig. 10c and d). In conclusion, it can be said that the amount of charge and particle size are both effective parameters, but more theoretical/experimental studies are needed to investigate the effect rate of each of these two parameters on the movement speed of nanomotors. Upon examination of the time interval investigated in this study, it can be observed that all samples exhibit enhanced diffusion behavior which follows a ballistic motion. The mean squared displacement (MSD) of the particles exhibits a non-linear increase with time, as evidenced by the relationship ⟨Δx 2 ⟩ ∼ Δt 2 . This phenomenon is indicative of superdiffusive transport, which occurs due to the propulsion of the particles under the influence of an electric field 39 .
The only item that revealed a high inconsistency with other data in Table 4 is related to nanomotors containing sodium ions. Because, the only cation present in sodium alginate-based nanomotors is sodium, while in nanomotors with other cations, in addition to the arrival ions (ions replaced instead of sodium), some departure ions (sodium ions) also exist. Therefore, the presence of extra salts beside the sodium ions increased the ionic concentration of the solution. As a result, the motor moving speed becomes higher when the ionic concentration rises due to the increase in the generated driving forces 37 . JNMs with electrical propulsion mechanisms combined with a magnetic field as an external source are prevalent, powerful, and highly efficient. Incorporating magnetic elements iron (III) oxide into JNMs allows for accurate motion control of nanomotors. We next evaluated the micromotor propulsion in magnetic mode. The locomotive behavior of Janus(Cs-Fe 3 O 4 -Na/Alg) nanomotors by an external magnetic field was recoded via camera (Video S13). A change in the trajectory with directional movement can be observed by applying the magnetic field (Fe 3 O 4 engine). The magnetic force changes the flow rate around the Janus micromotors, thereby increasing the acceleration of the micromotor, which could be very useful for future applications in complex media. It should be emphasized that the remarkably high velocity exhibited by these nanomotors posed a significant challenge in terms of accurately tracking their movements and conducting meaningful MSD analysis.

Conclusions
Here, we report an electrically powered Janus micromotor based on SPION that moves in aqueous media. This Janus nanomotor consists of a uniform spherical SPION as the core and two hemispheres of Na/Alg and Cs as the shell. Based on the electroactive properties of sodium alginate, the micro/nanomotor not only moves in solution under the influence of an electric field based on the electroosmotic mechanism, but also shows the great versatility in regulating the movement behavior under the influences of the electrical field. The electrical field controls the motion direction and speed of Janus micro/nanomotor in aqueous media on-demand. In addition, we found that the movement speed of nanoparticles increases with increasing the voltage between the two electrodes. The addition of divalent and trivalent ions as crosslinking agents affects both the size of nanomotors and the total charge of the medium. Increasing the amount of either of these two parameters could increase the final speed of the nanomotors. Therefore, the final velocity observed for each nanoparticle is the cross-product of the vectors of these two parameters. The highest speed of JNMs is observed when Fe 3+ is used as crosslinking agent (about 7.2181 μm 2 /s). As well as, the addition of non-crosslinking LiCl and KCl salts to the aqueous media causes an enhancement in the propulsion of microswimmers because in the presence of these monovalent ions, the size of nanoparticles -as an influencing parameter on velocity-is much higher than di/trivalent ions. www.nature.com/scientificreports/